1. 1.0 ‑ 2.0 mL fluid (abdominal, pleural, or CSF) in EDTA (lavender top tube).
2. Fresh tissue biopsy of an affected organ or lymph node placed in a sterile tube with 0.5 mL saline.
3. Aspirated tissue (of an affected organ or lymph node) placed on slide(s).
Note: Formalin fixed tissue is NOT acceptable.
Test Description
This PCR test detects mRNA of the M gene of all known feline coronavirus strains in any sample. However, for diagnosis of FIP, only the detection of mRNA outside of the intestinal tract is indicative since active replication of the virus in circulating mononuclear cells is typical for FIP.
Note: Most common sample submission is peritoneal effusion that on fluid analysis is consistent with FIP. Other sample options include CSF, tissue, and tissue aspirates (lymph node, etc.).