INTRODUCING CADET® BRAF and CADET® BRAF-PLUS

A simple free-catch urine analysis system for non-invasive detection and monitoring of canine transitional cell carcinoma (TCC)/urothelial carcinoma (UC) – the most common form of canine bladder and prostate cancer. 
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Leaders in Molecular Diagnostics

We are pioneers, innovators and researchers – and we are also dog lovers. That’s why we are driven to understand the biological and environmental influences of canine cancer, to create solutions for accurate and early detection that will help to enhance the health and welfare of our canine companions. Our products feature forensic-level sensitivity and are supported by robust research, proven science and leading genetics and oncology experts.

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Background on TCC and UC

 

This year in the US, approximately 80,000 dogs will be diagnosed with canine transitional cell carcinoma (TCC) of the urinary tract, also known as urothelial carcinoma (UC). The disease affects the bladder, urethra, and kidneys of male and female dogs and also the prostate of males. Symptoms include straining to urinate, repeated frequent attempts to urinate, blood in the urine, and repeated bacterial infection.

TCC/UC accounts for an estimated 1-2% of all cancer cases diagnosed in dogs, and can affect any breed; however the disease shows remarkably elevated incidence in several popular breeds. These, when combined, account for over a third of all diagnosed TCC/UC cases in purebred dogs. 

 

High-risk breeds include the American Eskimo Dog, Australian Cattle Dog, Australian Shepherd, Beagle, Bichon Frise, Border Collie, Parson Russell Terrier, Lhasa Apso, Rat Terrier, Russell Terrier, Scottish Terrier, Shetland Sheepdog, West Highland White Terrier, and Wire Fox Terrier.


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How CADET BRAF is Used in a Clinical Setting

CADET BRAF evaluates urine samples from dogs for the presence of cells harboring the BRAF mutation. The assay identifies the 85% of TCC/UC cases that are associated with the BRAF mutation.
 
The forensic level of detection of the assay can detect as few as 10 mutation-bearing cells in a urine sample and so is able to detect the presence of a developing TCC/UC, often several months before any advanced clinical signs associated with the cancer become evident. This enables owners and veterinarians to initiate appropriate treatment very early in the course of the disease, potentially before the mass has become invasive.  Dogs already presenting with urinary symptoms that may be caused by a TCC/UC can be evaluated for the presence of a TCC/UC simply by submitting a urine sample to Antech Diagnostics.

Additionally, the assay offers a sensitive means to monitor BRAF-mutant TCC/UC cases during the course of their treatment, for therapeutic response and relapse.  We have developed CADET BRAF exclusively for expedited assessment of dogs displaying symptoms consistent with TCC/UC, and for cases undergoing treatment.

CADET BRAF-PLUS

CADET BRAF-PLUS provides further evaluation of those dogs that present with clinical signs consistent with TCC/UC but for which no BRAF mutation is detected. CADET BRAF-PLUS increases the sensitivity for detection of canine bladder and prostate cancers to >95 %.
 
If the specimen is eligible, Antech will automatically run CADET BRAF-PLUS.
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Frequently Asked Questions

What is the procedure for collecting and shipping urine samples to the testing laboratory?
You can download instructions here. 

Do you have any recommendations on how to collect a urine sample? 
Use a clean household container to collect urine from the dog. We suggest you use a single-use, disposable container, cup or tray; however a disposable plastic tub, ladle or similar container is suitable as long as it is well sanitized between uses, especially if you are collecting samples from different dogs. This is to ensure that urine from one dog does not contaminate that of another dog.   

What should I do if the dog does not produce enough urine to fill the sample container to the line?
It is not essential that the required volume of urine is collected at one single time.