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Individual Tests
  Code Test Description
* Indicates send out test
  T645 IgA
  Specimen 1 ml serum or plasma
  Method RID
  Schedule 1–3 days
  T646 IgG Subclass A
  Specimen 1 ml serum or plasma
  Method RID
  Schedule 5–7 days
  T647 IgG Subclass B
  Specimen 1 ml serum or plasma
  Method IRID
  Schedule 5–7 days
  T090 IgG (Equine IgG Total)
  Specimen 1 ml serum or plasma
  Method Immunoturbidimetric Nephelometry
  Schedule 1–3 days
  Comments This is preferred test for accurate assessment of failure of passive transfer.
  S16435 IgG Subclass T
  Specimen 1 ml serum or plasma
  Schedule RID
  Schedule 5–7 days
  T656 IgM
  Specimen 1 ml serum or plasma
  Method RID
  Schedule 1–3 days
  * S16510 Leptospirosis Panel
  Specimen 1 ml serum or 1 ml plasma
  Method Micro-agglutination (MAT)
  Schedule 5–10 days
  Indication Tests for the presence of antibodies to L. canicola, L. icterohaemorrhagica, L. grippotyphosa, L. hardjo, L. pomona, L. autumanalis, and L. bratislava.
  Interpretive
  Guidelines		 Titers ò 1:250 suggest recent or current infection. A negative antibody result does not rule out leptospirosis. During the acute phase, there may be little to no detectable antibody. If clinical signs persist, suggest retesting in 2 weeks. Vaccination can cause positive titers.
  * S16514 Leptospira PCR
  Specimen 3 ml urine or unfixed kidney tissue
  Method PCR
  Schedule 7–14 days
  Comments Leptospirosis organisms are shed in the urine intermittently and so can be missed when a single sample is tested.
  Submission
  Instructions
  1. 5–10 ml from 3 to 4 different days
  2. Spin down, pour off supernate, suspend sediment in normal saline (1 – 2 ml), refrigerate each sample.
  3. Pool samples and send on ice.
  T672 Lyme IgG, Equine
  Specimen 1 ml serum or CSF
  Method IFA
  Schedule 1–4 days
  Indication Possible exposure to Borrelia burgdorferi.
  Interpretive
  Guidelines		 A titer >/= 1:64 suggests exposure or active infection. If result is borderline, we recommend retesting in 3 to 4 weeks. Vaccination can give a positive result.
  S16836 Lyme Disease Western Blot
  Specimen 1 ml serum or CSF
  Method Western Blot
  Schedule 2–7 days
  Indication Distinguish between natural exposure to and vaccination with Borrelia burgdorferi.
  S16270 Neorickettsia Risticii AB
(Ehrlichia Risticii)
(Potomac Horse Fever)
(Equine Monocytic Ehrlichiosis AB)
  Specimen 1 ml serum
  Method IFA
  Schedule 3–5 days
  Indication Measures qualitative antibody levels to Neorickettsia risticii the causative agent of Potomac Horse Fever.
  * S16705 Rhodococcus Equi AB
  Specimen 1 ml serum
  Method ELISA or Hemagglutination inhibition
  Schedule 5–10 days
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