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Endocrinology

  Code Test Description
* Indicates send out test
  T447 Dexamethosone Suppression Test (2 samples)
  Specimen 1 ml separated serum or heparinized plasma for each pre and post
  Method RIA
  Schedule 1–2 days
  Indication Dexamethasone suppression series are used to help identify pituitary adenomas of the pars intermedia.
  Special Drawing
  Instructions
OVERNIGHT DEXAMETHASONE SUPPRESSION (2 Samples)

Day One – pre PM sample (after 5:00 PM) administer 40mg Dexamethasone Sodium Phosphate IM

Day Two – post AM sample (9:00 AM)
  S16295 Estradiol
  Specimen 1 ml serum
  Method RIA
  Schedule 3–7 days
  Comments Run after 120 post breeding +/- progesterone level (See page 20 and the reproductive testing chart for testing guidelines.)
  *S16300 Estrone Sulfate
  Specimen 1 ml serum
  Method ELISA
  Schedule 7–10 days
  Indication Estrone sulfate is a hormone produced by the fetoplacental unit. It is an indication of pregnancy and fetal well being.
  Comments This test is diagnostic for equines only. Collect serum at least 100 days after breeding.
  *S4131 Inhibin
  Specimen 1 ml frozen or cold serum (send to the lab on ice)
  Schedule 3 weeks
  T470 Insulin w/ Glucose
  Tests Insulin, Glucose
  Method Insulin level obtained by radioimmunoassay. Glucose level obtained by enzymatic method.
  Specimen 1 ml serum or heparinized plasma separated immediately from cells
  Schedule 1–4 days
  Comments Elevated insulin levels in the face of euglycemia supports insulin resistance and antagonism commonly observed with adenomas of the pars intermedia lobe of the pituitary gland.
  S16370 Insulin-Like Growth Factor-1 (IGF-1)
  Specimen 2 ml plasma from plastic LT.
  Method RIA
  Schedule 7–10 days
  Comments Use chilled plastic LT containing for sample collection, centrifuge immediately and transfer plasma to a plastic vial. Transport on ice or cold packs. Do not use any plain, uncoated glass tubes or pipettes.

IGF-1 levels parallel growth hormone levels and are used as an indicator of insulin resistance and growth hormone responsive reproductive disorders.
  S16595 Parathyroid Hormone and Ionized Calcium Panel
  Specimen PTH - 1 ml frozen or cold serum (send to the lab on ice)

Ionized Calcium - 1 ml serum. Anaerobically transfer serum from a spun SST or RT (use a needle and syringe to avoid air exposure) into a plain RT. Please boldly label the RT as "Serum" and freeze. Send to lab on ice packs.
  Method RIA
  Schedule 3–5 days
  Comments PTH levels are used to evaluate calcium homeostasis, primary hyperparathyroidism, secondary renal disease/hyperparathyroidism, hypercalcemia and hypocalcemia.

Ionized Calcium can be artifactually decreased (Ca2+) if exposed to air when drawn. The test can also be artifactually increased (Ca2+) if transported by SST.
  S16635 PMSG, Pregnant Mare Serum Gonadotrophin
  Specimen 2 ml serumPlasma from LT containing Aprotinin.
  Method ELISA
  Schedule 3–7 days
  Comments Run on days 45–120 post breeding in conjunction with progesterone levels. (See page 20 and the reproductive testing chart for testing guidelines.)
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