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August • 2004
 
 
Lab Tip
 
WEST NILE VIRUS TESTS FOR HORSES & DOGS
Test Test Code Sample Comments
West Nile Virus IgM Capture ELISA 85624 1 ml serum (spun SST or RTT). Turn-around time is 2-3 days
WEST NILE VIRUS TESTS FOR AVIANS & OTHER SPECIES
West Nile Virus Titer 85448 1 spun GRT (with gel) or 1 spun SST.

Minimum sample volume is 100 µl of plasma or serum.
This is a serum neutralization (SN) test and detects predominantly IgG. A positive SN titer may be due to current infection, previous infection, or vaccination. A negative SN titer may occur with acute infections, or failure of the bird to mount an appropriate IgG response. Turn-around time is 10-14 days.
West Nile Virus PCR Test 85449 1 unspun GRT (± gel) or LTT. Minimum sample volume is 200 µl of whole blood.

Tissue samples—kidney (preferred), heart, or brain in a sterile RTT.
Turn-around time 5-7 days.
West Nile Virus Isolation 85450 Kidney, brain, or heart in a sterile RTT. Turn-around time is 3-4 weeks.
 
 
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